Why many 3D cell culture models remain hard to trust

Most 3D cell culture setups still do not reflect tissue-like oxygen conditions and are difficult to standardize across experiments.

Unrealistic Oxygen Environments

Cells are typically cultured at ambient “air oxygen”. The actual oxygen levels around 3D structures are rarely measured and often unknown.

Fragmented Workflows

One system for organoid formation, another for assays, a third for imaging – organoids are transferred between plates, introducing variability and manual effort.

Limited throughput and reproducibility

Organoid size and shape often vary, making it challenging to generate robust, comparable datasets for decision-making.

Would you trust a drug tested under non-physiological conditions? CaviSphere addresses these issues by standardizing 3D culture in defined microcavities and enabling a straightforward path to oxygen-sensing – without replacing your existing instruments.